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产品概述
名称 | HuH-7 (人肝癌细胞) (STR鉴定正确) |
别称 | HuH-7; HUH-7; HuH7; Huh7; HUH7; JTC-39; Japanese Tissue Culture-39 |
种属 | 人 |
年龄(性别) | 男 |
组织来源 | 肝;肝癌 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | 据说,HuH-7细胞可产甲胎蛋白、胰酶α抗体、血浆铜蓝蛋白、纤维蛋白原、纤维粘连蛋白等。 |
生长培养基 | DMEM(PM150210)+10% FBS(164210-500)+1% P/S(PB180120) |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2~3次/周 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
保藏机构 | CCLV; CCLV-RIE 1079 JCRB; JCRB0403 KCB; KCB 200970YJ KCLB; 60104 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11 D2S1338 19 D3S1358 15 D5S818 12 D7S820 11 D8S1179 14,15 D13S317 10,11 D16S539 10 D18S51 15 D19S433 13,14 D21S11 30 FGA 22 PentaD 12 PentaE 11 TH01 7 TPOX 8,11 vWA 16,18 D1S1656 16 D6S1043 13,15 D12S391 20,21 -
STR鉴定图
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参考文献
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Tanshinone I induces cell apoptosis by reactive oxygen species-mediated endoplasmic reticulum stress and by suppressing p53/DRAM-mediated autophagy in human hepatocellular carcinoma(2020.02.04)
ZUOZHE:Xu Liu, JiKui Liu
QIKAN:Artificial Cells, Nanomedicine, and Biotechnology
DOI:
YINGXIANGYINZI :3.343
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18-GA-Suc Modified Liposome Loading Cantharidin for Augmenting Hepatic Specificity: Preparation, Characterization, Antitumor Effects, and Liver-Targeting Efficiency(2020.06)
ZUOZHE:Kun Zhu, Lili Zhou, Manshu Zou
QIKAN:Journal of Pharmaceutical Sciences
DOI:
YINGXIANGYINZI :2.997
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Circular RNA circ_0091579 Promotes Hepatocellular Carcinoma Proliferation, Migration, Invasion, and Glycolysis Through miR-490-5p/CASC3 Axis(2020.07.14)
ZUOZHE:Wei Liu, Changbin Yin, and Yue Liu
QIKAN:Cancer Biotherapy & Radiopharmaceuticals
DOI:
YINGXIANGYINZI :2.314
引用产品:CL-0120
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Complement factor H‑related 3 overexpression affects hepatocellular carcinoma proliferation and apoptosis(2018.05.11)
ZUOZHE:Hong Liu, Ligang Zhang, Pengyan Wang
QIKAN:Molecular Medicine Reports
DOI:
YINGXIANGYINZI :2.1
引用产品:CL-0120