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XIBAOXIZUOWEIZHENGCHANGHUOZHONGLIUZUZHIDEMOSHIXIBAOBEIGUANGFANYINGYONGYUKEXUEYANJIUHEYAOWUKAIFA,RANERHENDAYIBUFENDEXIBAOXIBEICUOWUBIAOJIHUOBEIQITAGETI、ZUZHI、ZHONGXILAIYUANDEXIBAOSUOTIDAI。

SUIZHEYUELAIYUEDUODEDINGJIZAZHISHEDUOCIFAWENHUYUZHONGSHIXIBAOJIAOCHAWURANJICUOWUBIANSHIWENTI,XUANZEZHENGQUEZHENSHIDEXIBAODEZHONGYAOXINGRIQUTISHENG,KEYANGONGZUOZHEZAIGOUMAIXIBAOQIANYIDINGYAOZAISANSILV,DUOFANGXUNZHAO,XUANZEHAODEXIBAOGONGYINGSHANG,XUANZENENGTIGONGXIBAOXIJIANDINGBAOGAODEXIBAOGONGYINGZHE,FOUZEJISHINIFUCHUZAIDUODENULIYEDOUSHIBAIBAIDEFUZHUDONGLIU。

为什么要进行细胞系鉴定?

HENDUOSHENGWUSHENGWUYIXUELINGYUDEYANJIUDOUCAIYONGPEIYANGXIBAOLAIJINXING,ZHEIXIEXIBAOKENENGSHICONGXIBAOKU(RUMEIGUOATCC,American Type Culture Collection)DELAI,YEKENENGSHISHOUZENGYUQITAYANJIURENYUAN;YOUQIZAIZHONGGUO,MUQIANXIBAODECHUANBOTAIFUZA、WUXU,HENDUODOUBUSHITONGGUOZHENGGUITUJINGHUODE,RUYOUQINGZENGSONGDENG。

JUTONGJI,YUEYOU30%XIBAOXIBEIJIAOCHAWURANHUOCUOWUBIANSHI,YINSHIYONGLEJIAOCHAWURANHUOCUOWUBIANSHIDEXIBAOHUIDAOZHIYANJIUJIELUNCUOWU、JIEGUOBUKEZHONGFU、LINCHUANGXIBAOZHILIAOSHIBAIDENG。BUJINLANGFEIDALIANGSHIJIAN、JINGLIHEJINQIAN,HAIKENENGZAOCHENGBUKEWANHUIDEZAINANXINGHOUGUO。WEICI,HENDUOXIBAOKUXIANZAIDOUYAODUITIJIAOLAIDEXIBAOXIJINXINGJIANDING,BINGDUIXIBAOXIZHIJIANDEJIAOCHAWURANJINXINGJIANCE。

GEZHONGZAZHIDUOCIFACHUHUYU,YAOQIUYANJIUZHEDUIXIBAOJINXINGJIANDING,NIHDENGQUANWEIJIGOUYEJIANGXIBAOXIJIANDINGZUOWEIJIJINSHENQINGDEQIANTITIAOJIAN。

人源细胞的鉴定——STR鉴定

STR(Short Tandem Repeat,DUANCHUANLIANZHONGFUXULIE)JIYINFENXINGYIBEIICLAC、ATCCDENGQUANWEIJIGOUZUOWEIJINBIAOZHUNYINGYONGYUXIBAOJIANDING,MUQIANZHUYAOJIYUGUOJISHENFENRENZHENGWEIYUANHUIDEBIAOZHUN。YIJUGAIBIAOZHUN,KEYITONGGUODUOZHONGYINGGUANGPCRJISHU,DUIRENYUAN8GESTRWEIDIANYIJI1GEXINGBIEJUEDINGWEIDIANJINXINGJIANCE。

非人源细胞的鉴定——种属鉴定

STRJIANDINGYIJINGCHENGWEIXIBAOSHENFENQUERENDEJINBIAOZHUN,DANSHISTRJIANDINGMUQIANZHISHIYONGYURENJIXIAOSHUDENGSHAOSHUJIGEZHONGSHUDEXIBAO,NEIMEDUIYUFEIRENYUANDEXIBAOWOMENYINGGAIRUHEQUJIANDINGNI?

TONGCHANGQINGKUANGXIA,DUIYUFEIRENYUANXIBAO,WOMENSHOUXIANYAOJIEJUEDESHIJIAOCHAWURANDEWENTI,QUEBAOSHIYONGDEXIBAOSHISUOXUYAODEDANYILAIYUANDEXIBAO。ERJIEJUEDEFANGFAJIUSHIXIBAODEZHONGSHUJIANDING,MUQIANCHANGJIANDEXIBAOZHONGSHUJIANDINGFANGFAYOURANSETIFENXI、TONGGONGMEIFENXI、DNATIAOMACEXUJIPCRFADENG。ZHEIJIZHONGFANGFAZHONG,ZUICHANGYONG、ZUIPUBIANDEWEIPCRFA。

PCR法鉴定细胞来源种属

方法一:

ZHENDUICHANGYONGZHONGSHUDEXIBAO(REN、DASHU、XIAOSHU),SHIYONGZHONGSHUTEYIXINGDEYINWUJINXINGPCRKUOZENG,YONG1.5%QIONGZHITANGNINGJIAODIANYONGJIANCEMUDETIAODAI,LAIPANDUANXIBAODEZHONGSHUJISHIFOUCUNZAIBUTONGZHONGSHULAIYUANDEXIBAOJIAOCHAWURAN。

鉴定细胞来源种属

ZUOTUWEIBAOHANRENYUANXIBAOWURANDEXIAOSHUXIBAO;YOUTUWEIDANYILAIYUANDEXIAOSHUXIBAO

方法二:

TIQUXIBAODNA,YONGZHONGSHUFUHEYINWUMIXDUIDONGWUXIANLITICOXIJIYINKUOZENG,RANHOUYONGYICHUANFENXIYIDUIKUOZENGCHANWUJINXINGMAOXIGUANDIANYONGJIANCEHEFENXI。

鉴定细胞来源种属2

鉴定细胞来源种属3

KUOZENGJIEGUOXIANSHIWEIDASHUXIBAO,QIEWUQITAZHONGSHUXIBAOJIAOCHAWURAN

种属鉴定结果判定一般包含两项:是否为单一种属来源细胞以及来源细胞种属

CHULEBIYAODEZHONGSHUJIANDINGWAI,ZAISHIYONGXIBAOZHIQIANKEZHENDUIZISHENXUQIUDUIXIBAOJINXINGXIANGGUANXIBAOTEXINGDEJIANDING,RUMIANYIYINGGUANGHUOLIUSHIJIANCEDENG。

普诺赛(Procell)是一家专注于研发和生产原代细胞、肿瘤细胞及细胞培养相关试剂产品的生物高科技企业,可以为各类研究机构提供符合标准规范的原代细胞、肿瘤细胞及相关实验技术服务。

公司已对公司网站上提供的所有人源细胞系进行全面的STR鉴定,经STR鉴定通过的细胞系的鉴定报告将免费提供给所有下单订购细胞的客户!

此外,普诺赛所有在售大鼠细胞系,均应用PCR扩增→凝胶电泳跑胶进行种属鉴定;对于所有在售小鼠细胞系,除了极少数采用PCR扩增→凝胶电泳跑胶之外,其它均应用STR鉴定,以判断培养细胞是否存在种属间的交叉污染,为您的实验保驾护航。

普诺赛将始终确保为客户提供的细胞具有真实性、正确性,无交叉污染,消除客户因购买细胞而产生的质量和真假的后顾之忧,以最大的诚意提供信息最全面的细胞产品。

张晓丽

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